CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein-9) system, discovered in 2012 by the 2020 Nobel Prize-winning Laureates Jennifer Doudna and Emmanuelle Charpentier is a bacterial defensive mechanism that exhibits the cleavage of genomic DNA at the desired location, resulting in the exit of the old genes and the induction of a new set of genes. The accuracy, precision or fidelity of the genetic cut depends on the target and the proto-spacer adjacent motif (PAM) sequences. The Cas9 protein recognises the PAM sequence (5'-NGG-3') by selecting the correct location of base-pair bonds within the target sequence on the host genome. Assembling the nucleotide sequence related to PAM and target sequence into a plasmid and then transfecting the plasmid into a cell shows that Cas9 with the help of a crRNA detected the correct sequence within a host cell. This results in a single or double-stranded break at the appropriate location in the DNA, thereby working as a molecular scissor and performing a genetic cut. We choreographed this tool in achieving the information related to the generation of the PAM sequence and the off-target sites associated with the EGFR gene. We have identified the top 4 best gRNA sequences based on the highest SYNTHEGO scores range 0.98 to 0.99. Furthermore, we used CCTop to identify the 4 best targets and guide RNA sequences with the highest efficacy score. Our manuscript is aimed at showcasing the best target sequence utilizing model software like that of SYNTHEGO and CCTop.